Modules#

Kleborate v3 includes a range of modules for typing bacterial genomes, most of which are specific to a particular species or complex (currently Klebsiella pneumoniae species complex, Klebsiella oxytoca species complex, Escherichia coli/Shigella). We therefore recommend specifying --preset (kpsc, kosc, escherichia) or --modules (list of modules to run based on the organism). This will run the species detection module first, and if the species matches the expected preset or module, the modules will be run (if a different species is called, Kleborate will report the species but no further typing will be conducted and the remaining fields will be blank).

Kleborate modules are divided into:

  1. General Modules

  2. Modules for Klebsiella pneumoniae species complex

  3. Modules for Klebsiella oxytoca species complex

  4. Modules for Escherichia coli/Shigella

Summary of availabe modules and their output columns#

Module Name

Columns

enterobacterales__species

species, species_match

general__contig_stats

contig_count, N50, largest_contig, total_size, ambiguous_bases, QC_warnings

kpsc__mlst

ST, gapA, infB, mdh, pgi, phoE, rpoB, tonB

klebsiella__ybst

YbST, Yersiniabactin, ybtS, ybtX, ybtQ, ybtP, ybtA, irp2, irp1, ybtU, ybtT, ybtE, fyuA

klebsiella__cbst

CbST, Colibactin, clbA, clbB, clbC, clbD, clbE, clbF, clbG, clbH, clbI, clbL, clbM, clbN, clbO, clbP, clbQ

klebsiella__abst

AbST, Aerobactin, iucA, iucB, iucC, iucD, iutA

klebsiella__smst

Salmochelin, SmST, iroB, iroC, iroD, iroN

klebsiella__rmst

RmST, RmpADC, rmpA, rmpD, rmpC

klebsiella__rmpa2

rmpA2

kpsc__virulence_score

virulence_score (Score of 0-5)

kpsc__amr

AGly_acquired, Col_acquired, Fcyn_acquired, Flq_acquired, Gly_acquired, MLS_acquired, Phe_acquired, Rif_acquired, Sul_acquired, Tet_acquired, Tgc_acquired, Tmt_acquired, Bla_acquired, Bla_ESBL_acquired, Bla_ESBL_inhR_acquired, Bla_Carb_acquired, Bla_chr, SHV_mutations, Omp_mutations, Col_mutations, Flq_mutations, truncated_resistance_hits, spurious_resistance_hits

kpsc__resistance_score

resistance_score (Score of 0-3)

kpsc__resistance_gene_count

num_resistance_genes

kpsc__resistance_class_count

num_resistance_classes

kpsc__cipro_prediction

Ciprofloxacin_prediction, Ciprofloxacin_profile, Ciprofloxacin_profile_support, Ciprofloxacin_MIC_prediction

kpsc__wzi

wzi allele

kpsc__kaptive

K_locus, K_type, K_locus_confidence, K_locus_problems, K_locus_identity, K_Missing_expected_genes,O_locus, O_type, O_locus_confidence, O_locus_problems, O_locus_identity, O_Missing_expected_genes

kpsc__cgmlst

cgST, LIN code, Sublineage, Clonal group

kpsc__mrk

mrkST, mrkA, mrkB, mrkC, mrkD, mrkF, mrkH, mrkI, mrkJ

kosc__mlst

ST, gapA, infB, mdh, pgi, phoE, rpoB, tonB

kosc__kaptive

K_locus, K_type, K_locus_confidence, K_locus_problems, K_locus_identity, K_Missing_expected_genes, O_locus, O_type, O_locus_confidence, O_locus_problems, O_locus_identity, O_Missing_expected_genes

escherichia__mlst_pasteur

ST, dinB, icdA, pabB, polB, putP, trpA, trpB, uidA

escherichia__mlst_achtman

ST, adk, fumC, gyrB, icd, mdh, purA, recA

escherichia__pathovar

Pathotype, Stx1, Stx2, ST, LT, eae, ipaH

escherichia__mlst_lee

LEE_ST, LEE_lineage, LEE_eae, LEE_tir, LEE_espA, LEE_espB, LEE_espD, LEE_espH, LEE_espZ

escherichia__stxtyper

Stx_type, operon, identity, target_start, target_stop, target_strand, A_reference ‘A_identity, A_reference_subtype, A_coverage,B_reference, B_reference_subtype, B_identity, B_coverage

escherichia__ectyper

O-type, H-type, Serotype, QC, Evidence, GeneScores, AllelesKeys, GeneIdentities(%), GeneCoverages(%), GeneLengths, Warnings

escherichia__ezclermont

Clermont_type, Clermont_profile

escherichia__amr

Aminoglycoside, Fluoroquinolone, Fosfomycin, Sulfonamide, Tetracycline, Glycopeptide, Colistin, Phenicol, Macrolide, Rifamycin, Trimethoprim, BetaLactam, Carbapenem, Cephalosporin, Methicillin, Other Classes

escherichia__cgMLST

cgST, LIN code, Sublineage, Clonal group

escherichia__kaptive

K_locus, K_type, K_locus_confidence, K_locus_problems, K_locus_identity, K_Missing_expected_genes