Installation#
Dependencies#
Kleborate requires the following software and libraries to be installed and available in your path:
Install Kleborate#
Create a conda environment containing Kleborate dependencies:
conda create -n klebsiella_analysis --solver=libmamba \
-c conda-forge \
-c bioconda \
python=3.11 \
biopython=1.81 \
beautifulsoup4 \
numba \
minimap2 \
mash \
mist_typing \
ezclermont \
ectyper \
ncbi-stxtyper \
shigapass \
ncbi-amrfinderplus -y
Activate the environment and install kleborate (and Kaptive) using pip:
conda activate klebsiella_analysis
pip install kleborate==3.3.0b1
Quick installation check#
Download K. pneumoniae genome and run kpsc__mlst module
wget https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/002/813/595/GCF_002813595.1_ASM281359v1/GCF_002813595.1_ASM281359v1_genomic.fna.gz
kleborate -a GCF_002813595.1_ASM281359v1_genomic.fna.gz -o kleborate_test -m kpsc__mlst --trim_headers
Database set up#
Before running Kleborate, set up the reference databases required
KpSC cgMLST database (MiST)#
NOTE: KpSC cgMLST is only used by the module -m kpsc__cgmlst and preset -p kpsc, so if you are not analysing K. pneumoniae species complex you don’t need to do this step.
The kpsc__cgmlst module performs core-genome MLST (cgMLST) allele calling using MiST and uses the scgMLST629_S scheme hosted on the Institut Pasteur BIGSdb instance.
This requires a local, indexed copy of the cgMLST scheme to be stored in kleborate/modules/kpsc__cgmlst/data. As the scheme is under license, we cannot distribute it with Kleborate, and you will need to download the latest version yourself. To simplify this, we have supplied the Python script setup_cgmlst.py download and index the scheme database:
python setup_cgmlst.py
Note
Downloading profiles can take a long time due to the large number of entries.
Prerequisites#
Before running the setup_cgmlst.py script, ensure the following tools are installed and available in your PATH:
MiST (
mist)bigsdb-downloader (Required for authenticated Pasteur downloads:
pip install bigsdb-downloader)
What the script does#
Verifies Dependencies: Confirms
mistis accessible in your environmentInstalls
bigsdb-downloaderLocates Kleborate data path (
kleborate/modules/kpsc__cgmlst/data)Downloads the
scgMLST629_Sscheme from the Institut Pasteur BIGSdb instance
Download modes#
When running setup_cgmlst.py, you will be prompted to select one of two download modes:
Standard download (Public) Pulls public scheme data (frozen on 2024-12-31) without requiring login credentials.
Latest Pasteur database (Authenticated) Pulls the most up-to-date scheme data directly from Institut Pasteur and requires OAuth authentication. Uses the
bigsdb_authdownloader with credentials stored in.bigsdb_tokens/.
Pasteur credential setup (Mode 2)#
If you select Mode 2 and valid tokens are not found in .bigsdb_tokens/access_tokens, the script initiates the OAuth setup:
Obtain API Client Credentials: Register for database access via the Institut Pasteur BIGSdb Portal.
Request an OAuth Client Key and Secret by emailing
bigsdb@pasteur.fr.
Run Authentication via the Setup Script and input API Keys: Enter your
Client IDandClient Secretat the terminal prompts.Authorize in Browser: Open the generated URL in your browser, log in to your Pasteur account, and copy the verification code.
Complete Verification: Paste the verification code into the terminal. Access tokens will be saved to
.bigsdb_tokens/, and subsequent runs will skip re-authentication.
Output files#
Upon completion, the following files will be downloaded inside the target folder:
kleb_scgmlst_s/— Raw scheme FASTA alleles andprofiles.tsv.kleb_scgmlst_s-index/— Indexed binary database used bymistduring Kleborate runs.
Test kpSC cgMLST module#
kleborate -a GCF_002813595.1_ASM281359v1_genomic.fna.gz -o kleborate_test_cgmlst -m kpsc__cgmlst --trim_headers
AMRFinderPlus database#
NOTE: AMRFinderPlus is only used by the module -m escherichia__amr and preset -p escherichia, so if you are not analysing E. coli/Shigella you don’t need to do this step.
Download the latest AMRFinderPlus database:
amrfinder -u
Verify the installation and database#
# Check the software and database versions
amrfinder --version
amrfinder -l
E. coli cgMLST database (MiST)#
NOTE: E. coli cgMLST is only used by the module -m escherichia__cgmlst and preset -p escherichia, so if you are not analysing E. coli/Shigella you don’t need to do this step.
Note
In this beta release, E. coli cgMLST is provided as an optional module and is not included in the default -p escherichia preset. To run it, specify -m escherichia__cgmlst.
The escherichia__cgmlst module performs core-genome MLST (cgMLST) allele uses the Escherichia.cgMLSTv1 scheme hosted on EnteroBase.
Run the setup_ecoli_cgmlst.py script to download and index the scheme:
python setup_ecoli_cgmlst.py
Output files#
The following files will be created inside the kleborate/modules/ecoli__cgmlst/data folder:
ecoli_cgmlst_v1/— Raw scheme FASTA alleles andprofiles.tsv.ecoli_cgmlst_v1-index/— Indexed binary database used bymistduring allele calling.
EnteroBase API token (required for E. coli LIN codes)#
EnteroBase schemes do not include LIN codes in the downloaded profiles file. To retrieve the LIN code for a matched cgST, this module queries EnteroBase’s API token
Please request API access to the EnteroBase E. coli database at https://enterobase.warwick.ac.uk/ Once, you have obtained the token run;
echo 'YOUR_TOKEN' > ./enterobase_token
chmod 600 ./enterobase_token
When running the module pass the --ecoli_entero_token path
kleborate \
-a *.fasta \
-o kleborate_results \
-m escherichia__cgmlst \
--trim_headers \
--ecoli_entero_token ./enterobase_token
Test E. coli cgMLST module#
Download E. coli genome and test cgMLST module
wget https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/001/559/655/GCA_001559655.1_ASM155965v1/GCA_001559655.1_ASM155965v1_genomic.fna.gz
kleborate -a GCA_001559655.1_ASM155965v1_genomic.fna.gz -o escherichia_test -m escherichia__cgmlst --trim_headers
Validation tests#
kpsc preset test#
To test that Kleborate is installed and working correctly, download the example genome assembly and run Kleborate using the -p kpsc:
wget https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/002/813/595/GCF_002813595.1_ASM281359v1/GCF_002813595.1_ASM281359v1_genomic.fna.gz
kleborate -a GCF_002813595.1_ASM281359v1_genomic.fna.gz -o kleborate_test -p kpsc --trim_headers
If the installation is successful, the analysis should complete without errors and generate the expected output files.
Additional tests#
Additional K. pneumoniae test datasets are provided to further validate your installation:
kleborate -a test/kpsc_test/data/*.fasta.gz -o kleborate_kpsc_test -p kpsc --trim_headers
The generated output should match the corresponding reference files located in
test/kpsc_test/example_output/:
test/kpsc_test/example_output/klebsiella_pneumo_complex_output.txt:
strain |
species |
species_match |
contig_count |
N50 |
largest_contig |
total_size |
GC_content |
ambiguous_bases |
QC_warnings |
ST |
gapA |
infB |
mdh |
pgi |
phoE |
rpoB |
tonB |
YbST |
Yersiniabactin |
ybtS |
ybtX |
ybtQ |
ybtP |
ybtA |
irp2 |
irp1 |
ybtU |
ybtT |
ybtE |
fyuA |
spurious_YbST |
CbST |
Colibactin |
clbA |
clbB |
clbC |
clbD |
clbE |
clbF |
clbG |
clbH |
clbI |
clbL |
clbM |
clbN |
clbO |
clbP |
clbQ |
spurious_CbST |
AbST |
Aerobactin |
iucA |
iucB |
iucC |
iucD |
iutA |
spurious_AbST |
SmST |
Salmochelin |
iroB |
iroC |
iroD |
iroN |
spurious_SmST |
RmST |
RmpADC |
RmpADC_status |
rmpA |
rmpD |
rmpC |
rmpA_promoter |
argR |
spurious_RmST |
virulence_score |
rmpA2 |
peg-344 |
AGly_acquired |
Col_acquired |
Fcyn_acquired |
Flq_acquired |
Gly_acquired |
MLS_acquired |
Phe_acquired |
Rif_acquired |
Sul_acquired |
Tet_acquired |
Tgc_acquired |
Tmt_acquired |
Bla_acquired |
Bla_inhR_acquired |
Bla_ESBL_acquired |
Bla_ESBL_inhR_acquired |
Bla_Carb_acquired |
Bla_chr |
SHV_mutations |
Omp_mutations |
Col_mutations |
Flq_mutations |
truncated_resistance_hits |
spurious_resistance_hits |
resistance_score |
num_resistance_classes |
num_resistance_genes |
Ciprofloxacin_prediction |
Ciprofloxacin_profile_support |
Ciprofloxacin_profile |
Ciprofloxacin_MIC_prediction |
wzi |
K_locus |
K_type |
K_locus_confidence |
K_locus_problems |
K_locus_identity |
K_Missing_expected_genes |
O_locus |
O_type |
O_locus_confidence |
O_locus_problems |
O_locus_identity |
O_Missing_expected_genes |
cgST |
LIN code |
Sublineage |
Clonal group |
mrkST |
mrkA |
mrkB |
mrkC |
mrkD |
mrkF |
mrkH |
mrkI |
mrkJ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
007_S09 |
Klebsiella pneumoniae |
strong |
274 |
56676 |
199420 |
5902084 |
56.66 |
no |
N50 |
ST11-1LV |
3 |
3 |
1 |
1 |
1 |
4 |
346-4LV |
ybt 0; ICEKp10 (truncated) |
48 |
15 |
5 |
94 |
60 |
181 |
200*-8% |
50*-78% |
11*-43% |
4 |
84* |
17-3LV |
clb 3 (truncated) |
2 |
10*-24% |
2 |
2 |
2 |
2 |
2 |
8 |
2 |
2*-40% |
2 |
2 |
2* |
2 |
2 |
0 |
0 |
0 |
present |
2 |
aac(6’)-Ib3^ |
catA1^ |
dfrA30* |
TEM-1^;OXA-1;OXA-2 |
CTX-M-2 |
NDM-1 |
SHV-11 |
SHV:p.L35Q |
ompK36:p.134_135insGD;ompK35:p.R115VfsTer30 |
mgrB:c.T72del;pmrB:p.R94AfsTer32 |
gyrA:p.S83I;parC:p.S80I |
sul1.v2?-76%;aac(3)-IIa.v1?-0%;aac(3)-IId*?-0%;catB3.v1?-70% |
3 |
8 |
8 |
nonwildtype R |
99.21% R (N=2147/2167) |
>1 QRDR, 0 PMQR, * aac(6`)-Ib-cr |
2 mg/L [2-4] |
wzi64 |
KL64 |
Capsule null |
Typeable |
! |
99.76% |
OL2α.1 |
O2α |
Typeable |
98.73% |
cgST766 |
0_0_105_0_0_0_9_0_0_0 |
SL258 |
Cannot be determined |
0 |
||||||||||||||||||||||||||||||||||||||||||||||||
2024187392 |
Klebsiella pneumoniae |
strong |
132 |
174550 |
459900 |
5682502 |
56.66 |
yes (20) |
ambiguous_bases |
ST383 |
2 |
6 |
1 |
3 |
8 |
1 |
18 |
183 |
ybt 9; ICEKp3 |
5 |
11 |
6 |
5 |
1 |
25 |
37 |
9 |
5 |
5 |
11 |
0 |
63 |
iuc 1 |
1 |
15 |
1 |
1 |
1 |
0 |
147 |
rmp 1; KpVP-1 |
ON |
27 |
2 |
2 |
11T |
present |
4 |
rmpA2_6*-47% |
present |
strB.v1;aph(3’)-Ia.v1^;aph(3’’)-Ib.v1*;aadA1.v2;aph(3’)-VI;armA;aac(6’)-Ib-AKT |
qnrS1 |
msrE;mrx;mphA;mphE.v2 |
sul1;sul2 |
tet(A).v1 |
dfrA5 |
CTX-M-15;CTX-M-14^ |
NDM-5;OXA-48 |
SHV-1 |
ompK36:p.135_136insD;ompK35:c.T106del |
mgrB:c.T50del |
gyrA:p.S83F;gyrA:p.D87N;parC:p.S80I |
OXA-9.v1*-42%;TEM-135.v1*?-0% |
catA1*?-0% |
3 |
9 |
20 |
nonwildtype R |
99.22% R (N=2424/2443) |
>0 QRDR, >0 PMQR, * aac(6`)-Ib-cr |
4 mg/L [4-4] |
KL30 |
K30 |
Typeable |
! |
97.84% |
OL2α.2 |
O2β |
Typeable |
99.21% |
cgST19975; cgST20666; cgST52869; cgST63343 |
0_0_87_5_0_3_1_0_0_0 |
SL383 |
CG383 |
ST2977 |
22 |
147 |
352 |
231 |
71 |
256 |
143 |
140 |
||||||||||||||||||||||||||||||||||||||||
SRR33580217 |
Klebsiella pneumoniae |
strong |
76 |
272064 |
1055808 |
5520554 |
57.3 |
no |
ST23 |
2 |
1 |
1 |
1 |
9 |
4 |
12 |
47-2LV |
ybt 1; ICEKp10 |
2 |
2 |
2 |
2* |
2 |
6* |
6 |
2 |
2 |
2 |
2 |
29-1LV |
clb 2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
3* |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
1 |
iuc 1 |
1 |
1 |
1 |
1 |
1 |
2 |
iro 1 |
1 |
4 |
1 |
1 |
26 |
rmp 1; KpVP-1 |
ON |
2 |
2 |
2 |
11T |
present |
5 |
rmpA2_5-54% |
present |
SHV-11^ |
SHV:p.L35Q |
0 |
0 |
0 |
wildtype S |
90.99% S (N=5168/5680) |
0^ QRDR, 0 PMQR, 0 aac(6`)-Ib-cr |
0.25 mg/L [0.25-0.25] |
wzi1 |
KL1 |
K1 |
Typeable |
99.83% |
OL2α.2 |
O2β |
Typeable |
99.45% |
cgST65856 |
0_0_429_0_44_1_19_0_0_0 |
SL23 |
CG23 |
ST9549 |
985 |
1 |
1 |
1 |
1 |
1 |
2 |
1 |
||||||||||||||||||||||||||||||||
SRR33580216 |
Klebsiella pneumoniae |
strong |
98 |
215497 |
539719 |
5596385 |
57.23 |
no |
ST23 |
2 |
1 |
1 |
1 |
9 |
4 |
12 |
46-1LV |
ybt 1; ICEKp10 |
2 |
2 |
2 |
2 |
2 |
36 |
6* |
2 |
2 |
2 |
2 |
29-1LV |
clb 2 |
2 |
2* |
2 |
2 |
2 |
2 |
2 |
3 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
1 |
iuc 1 |
1 |
1 |
1 |
1 |
1 |
2 |
iro 1 |
1 |
4 |
1 |
1 |
40 |
rmp 1; KpVP-1 |
ON |
2 |
3 |
2 |
11T |
present |
5 |
rmpA2_5-54% |
present |
SHV-11^ |
SHV:p.L35Q |
0 |
0 |
0 |
wildtype S |
90.99% S (N=5168/5680) |
0^ QRDR, 0 PMQR, 0 aac(6`)-Ib-cr |
0.25 mg/L [0.25-0.25] |
wzi1 |
KL1 |
K1 |
Typeable |
99.81% |
OL2α.2 |
O2β |
Typeable |
99.45% |
cgST65857 |
0_0_429_0_4_0_35_0_0_0 |
SL23 |
CG23 |
ST1 |
1 |
1 |
1 |
1 |
1 |
1 |
2 |
1 |
||||||||||||||||||||||||||||||||
SRR5386028 |
Klebsiella pneumoniae |
strong |
312 |
104936 |
348607 |
5604201 |
57.03 |
no |
ST13 |
2 |
3 |
1 |
1 |
10 |
1 |
19 |
267-1LV |
ybt 17; ICEKp10 |
6* |
15 |
5 |
13 |
11 |
28 |
26 |
2 |
11 |
4 |
2 |
19-1LV |
clb 3 |
2 |
10 |
2 |
2 |
2 |
4 |
2 |
8 |
2 |
2 |
2 |
2* |
2 |
2 |
2 |
0 |
0 |
0 |
present |
2 |
aph(3’)-Ia.v1^;aadA2.v1;aac(6’)-Ib-cr5^ |
aac(6’)-Ib-cr5^ |
mrx;mphA |
sul1 |
tet(D) |
dfrA12 |
TEM-1^;OXA-1 |
CTX-M-15;SHV-5 |
KPC-2 |
SHV:p.G238S;SHV:p.E240K |
ompK36:c.25C>T |
mgrB:p.K43Ter |
gyrA:p.S83F;gyrA:p.D87N;parC:p.S80I |
OXA-9.v1*-42% |
catB3.v1?-70% |
3 |
10 |
14 |
nonwildtype R |
99.21% R (N=2147/2167) |
>1 QRDR, 0 PMQR, * aac(6`)-Ib-cr |
2 mg/L [2-4] |
wzi40 |
KL3 |
K3 |
Typeable |
? |
99.89% |
OL2α.2 |
O2β |
Typeable |
99.13% |
cgST32525 |
0_0_98_0_11_2_0_1_1_0 |
SL13 |
CG13 |
ST633-1LV |
5 |
143 |
659 |
229 |
1 |
116 |
1 |
||||||||||||||||||||||||||||||||||||||
ERR14097885 |
Klebsiella pneumoniae |
strong |
140 |
245450 |
558119 |
5866622 |
56.56 |
no |
ST147 |
3 |
4 |
6 |
1 |
7 |
4 |
38 |
584-2LV |
ybt 9; ICEKp? |
5 |
11 |
6 |
5 |
1 |
293 |
318 |
9 |
5 |
142 |
11 |
0 |
63 |
iuc 1 |
1 |
15 |
1 |
1 |
1 |
0 |
147 |
rmp 1; KpVP-1 |
ON |
27 |
2 |
2 |
11T |
present |
4 |
rmpA2_6*-47% |
present |
aph(3’)-Ia.v1^;aadA1.v2;aph(3’)-VI;armA |
qnrS1 |
msrE;mrx*;mphA;mphE.v2 |
catB3.v2 |
arr-3 |
sul1;sul2 |
dfrA5;dfrA50* |
OXA-9.v1;OXA-1 |
CTX-M-15 |
NDM-5 |
SHV-11^ |
SHV:p.L35Q |
ompK36:p.134_135insGD;ompK35:p.R60LfsTer31 |
pmrB:c.G445del |
gyrA:p.S83I;parC:p.S80I |
TEM-150*?-0%;aac(6’)-Ib-AKT.v3?-89% |
3 |
11 |
19 |
nonwildtype R |
99.22% R (N=2424/2443) |
>0 QRDR, >0 PMQR, * aac(6`)-Ib-cr |
4 mg/L [4-4] |
wzi64 |
KL64 |
K64 |
Typeable |
99.89% |
OL2α.1 |
O2α |
Typeable |
98.73% |
cgST10000; cgST11351; cgST35980; cgST65374 |
0_0_197_0_4_1_0_7_15_0 |
SL147 |
CG147 |
ST8-1LV |
6 |
3 |
2 |
12 |
8 |
7 |
15 |
test/kpsc_test/example_output/klebsiella_pneumo_complex_hAMRonization_output.txt:
Input File Name |
Gene Symbol |
Gene Name |
Nucleotide mutation |
Amino acid mutation |
Genetic Variation Type |
Drug Class |
Input Sequence ID |
Input Gene Length |
Input Gene Start |
Input Gene Stop |
Reference Gene Length |
Reference Gene Start |
Reference Gene Stop |
Sequence Identity |
Coverage |
Reference Accession |
Strand Orientation |
Analysis Software Name |
Analysis Software Version |
Reference Database Name |
Reference Database Version |
Input Protein Length |
Reference Protein Length |
Input Protein Start |
Input Protein Stop |
Reference Protein Start |
Reference Protein Stop |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
007_S09 |
aac(6)-Ib3 |
aac(6)-Ib3 |
Gene presence detected |
aminoglycoside antibiotic |
assembly_contig_237 |
654 |
67 |
621 |
555 |
1 |
555 |
99.64 |
100.00 |
NG_051438.1:20-574 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
catA1 |
catA1 |
Gene presence detected |
phenicol antibiotic |
assembly_contig_211 |
1493 |
223 |
882 |
660 |
1 |
660 |
99.85 |
100.00 |
NG_047582.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
dfrA30 |
dfrA30 |
Gene presence detected |
trimethoprim antibiotic |
assembly_contig_224 |
940 |
212 |
685 |
474 |
1 |
474 |
99.58 |
100.00 |
NG_047727.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
TEM-1 |
TEM-1 |
Gene presence detected |
penicillin beta-lactam |
assembly_contig_202 |
1745 |
37 |
897 |
861 |
1 |
861 |
99.88 |
100.00 |
AY263331.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
OXA-1 |
OXA-1 |
Gene presence detected |
penicillin beta-lactam |
assembly_contig_213 |
1474 |
65 |
895 |
831 |
1 |
831 |
100.00 |
100.00 |
NG_049392.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
OXA-2 |
OXA-2 |
Gene presence detected |
penicillin beta-lactam |
assembly_contig_223 |
940 |
98 |
925 |
828 |
1 |
828 |
100.00 |
100.00 |
NG_049496.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
CTX-M-2 |
CTX-M-2 |
Gene presence detected |
third-generation cephalosporin |
assembly_contig_166 |
4341 |
1046 |
1921 |
876 |
1 |
876 |
100.00 |
100.00 |
NG_048968.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
NDM-1 |
NDM-1 |
Gene presence detected |
carbapenem |
assembly_contig_156 |
5363 |
4188 |
5000 |
813 |
1 |
813 |
100.00 |
100.00 |
NG_049326.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
SHV-11 |
SHV-11 |
Gene presence detected |
penicillin beta-lactam |
assembly_contig_33 |
56676 |
27911 |
28771 |
861 |
1 |
861 |
100.00 |
100.00 |
CP168408.1:2797577-2798437 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
sul1.v2 |
sul1.v2 |
Gene presence detected |
sulfonamide antibiotic |
assembly_contig_233 |
694 |
1 |
635 |
840 |
1 |
635 |
100.00 |
75.60 |
NG_048081.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
aac(3)-IIa.v1 |
aac(3)-IIa.v1 |
Gene presence detected |
aminoglycoside antibiotic |
assembly_contig_225 |
913 |
1 |
639 |
861 |
223 |
861 |
100.00 |
74.22 |
NG_047244.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
aac(3)-IId |
aac(3)-IId |
Gene presence detected |
aminoglycoside antibiotic |
assembly_contig_205 |
1685 |
1 |
639 |
861 |
223 |
861 |
99.84 |
74.22 |
EU022314.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
catB3.v1 |
catB3.v1 |
Gene presence detected |
phenicol antibiotic |
assembly_contig_213 |
1474 |
1033 |
1474 |
633 |
1 |
442 |
100.00 |
69.83 |
NG_047605.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
blaSHV |
blaSHV |
p.L35Q |
Protein variant detected |
penicillin beta-lactam |
assembly_contig_33 |
56676 |
27911 |
28771 |
861 |
1 |
861 |
100.00 |
100.00 |
CP067381.1:82914-84074 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
007_S09 |
ompK36 |
ompK36 |
p.134_135insGD |
Inactivating mutation detected |
assembly_contig_11 |
96727 |
33833 |
34942 |
1104 |
1 |
1104 |
99.46 |
100.54 |
NC_016845.1:3727882-3728985 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
007_S09 |
ompK35 |
ompK35 |
p.R115VfsTer30 |
Inactivating mutation detected |
assembly_contig_14 |
91113 |
88694 |
89772 |
1080 |
1 |
1080 |
99.91 |
40.11 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||||
007_S09 |
mgrB |
mgrB |
c.T72del |
Inactivating mutation detected |
peptide antibiotic |
assembly_contig_101 |
18945 |
18872 |
18945 |
144 |
1 |
74 |
100.00 |
51.06 |
NC_016845.1:3337154-3337297 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
007_S09 |
pmrB |
pmrB |
p.R94AfsTer32 |
Inactivating mutation detected |
peptide antibiotic |
assembly_contig_19 |
78336 |
67548 |
68644 |
1098 |
1 |
1098 |
99.54 |
34.25 |
NC_016845.1:1694393-1695491 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
007_S09 |
gyrA |
gyrA |
p.S83I |
Protein variant detected |
fluoroquinolone antibiotic |
assembly_contig_11 |
96727 |
44326 |
44686 |
360 |
1 |
360 |
99.44 |
100.00 |
HM560971.1:119269-119072 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
120 |
120 |
1 |
120 |
1 |
120 |
||
007_S09 |
parC |
parC |
p.S80I |
Protein variant detected |
fluoroquinolone antibiotic |
assembly_contig_10 |
96805 |
29927 |
30287 |
360 |
1 |
360 |
99.72 |
100.00 |
HM560971.1:119269-119072 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
120 |
120 |
1 |
120 |
1 |
120 |
||
2024187392 |
strB.v1 |
strB.v1 |
Gene presence detected |
aminoglycoside antibiotic |
45_pilon |
15773 |
13554 |
14390 |
837 |
1 |
837 |
100.00 |
100.00 |
NG_047464.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
aph(3)-Ia.v1 |
aph(3)-Ia.v1 |
Gene presence detected |
aminoglycoside antibiotic |
94_pilon |
1056 |
67 |
882 |
816 |
1 |
816 |
99.75 |
100.00 |
CP157740.1:12584-13348 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
aph(3)-Ib.v1 |
aph(3)-Ib.v1 |
Gene presence detected |
aminoglycoside antibiotic |
45_pilon |
15773 |
14390 |
15193 |
804 |
1 |
804 |
99.88 |
100.00 |
NG_047413.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
aadA1.v2 |
aadA1.v2 |
Gene presence detected |
aminoglycoside antibiotic |
74_pilon |
2416 |
659 |
1447 |
789 |
1 |
789 |
100.00 |
100.00 |
NG_047327.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
aph(3)-VI |
aph(3)-VI |
Gene presence detected |
aminoglycoside antibiotic |
48_pilon |
12362 |
5950 |
6729 |
780 |
1 |
780 |
100.00 |
100.00 |
NG_051730.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
armA |
armA |
Gene presence detected |
aminoglycoside antibiotic |
57_pilon |
7423 |
6437 |
7210 |
774 |
1 |
774 |
100.00 |
100.00 |
NG_047476.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
aac(6)-Ib-AKT |
aac(6)-Ib-AKT |
Gene presence detected |
aminoglycoside antibiotic |
74_pilon |
2416 |
35 |
589 |
555 |
1 |
555 |
100.00 |
100.00 |
NG_056043.1:5-559 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
qnrS1 |
qnrS1 |
Gene presence detected |
Fluoroquinolone antibiotic |
48_pilon |
12362 |
1416 |
2072 |
657 |
1 |
657 |
100.00 |
100.00 |
NG_050543.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
msrE |
msrE |
Gene presence detected |
macrolide antibiotic |
57_pilon |
7423 |
2663 |
4138 |
1476 |
1 |
1476 |
100.00 |
100.00 |
NG_048007.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
mrx |
mrx |
Gene presence detected |
macrolide antibiotic |
66_pilon |
4017 |
973 |
2211 |
1239 |
1 |
1239 |
100.00 |
100.00 |
LC613143.1:28805-30043 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
mphA |
mphA |
Gene presence detected |
macrolide antibiotic |
66_pilon |
4017 |
71 |
976 |
906 |
1 |
906 |
100.00 |
100.00 |
NG_047985.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
mphE.v2 |
mphE.v2 |
Gene presence detected |
macrolide antibiotic |
57_pilon |
7423 |
1723 |
2607 |
885 |
1 |
885 |
100.00 |
100.00 |
NG_064660.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
sul1 |
sul1 |
Gene presence detected |
sulfonamide antibiotic |
85_pilon |
1489 |
312 |
1151 |
840 |
1 |
840 |
100.00 |
100.00 |
NG_048082.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
sul2 |
sul2 |
Gene presence detected |
sulfonamide antibiotic |
60_pilon |
5091 |
3808 |
4623 |
816 |
1 |
816 |
100.00 |
100.00 |
NG_051852.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
tet(A).v1 |
tet(A).v1 |
Gene presence detected |
tetracycline antibiotic |
64_pilon |
4106 |
901 |
2100 |
1200 |
1 |
1200 |
100.00 |
100.00 |
NG_048154.1:101-1300 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
dfrA5 |
dfrA5 |
Gene presence detected |
trimethoprim antibiotic |
81_pilon |
1897 |
1351 |
1824 |
474 |
1 |
474 |
100.00 |
100.00 |
NG_051699.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
CTX-M-15 |
CTX-M-15 |
Gene presence detected |
third-generation cephalosporin |
80_pilon |
1902 |
981 |
1856 |
876 |
1 |
876 |
100.00 |
100.00 |
NG_048935.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
CTX-M-14 |
CTX-M-14 |
Gene presence detected |
third-generation cephalosporin |
88_pilon |
1256 |
341 |
1216 |
876 |
1 |
876 |
99.89 |
100.00 |
NG_048929.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
NDM-5 |
NDM-5 |
Gene presence detected |
carbapenem |
48_pilon |
12362 |
9162 |
9974 |
813 |
1 |
813 |
100.00 |
100.00 |
NG_049337.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
OXA-48 |
OXA-48 |
Gene presence detected |
carbapenem |
84_pilon |
1610 |
26 |
823 |
798 |
1 |
798 |
100.00 |
100.00 |
NG_049762.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
SHV-1 |
SHV-1 |
Gene presence detected |
penicillin beta-lactam |
14_pilon |
148667 |
119862 |
120722 |
861 |
1 |
861 |
100.00 |
100.00 |
CP165807.1:2737916-2738776 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
OXA-9.v1 |
OXA-9.v1 |
Gene presence detected |
penicillin beta-lactam |
74_pilon |
2416 |
1492 |
2331 |
840 |
1 |
840 |
99.88 |
100.00 |
CP067618.1:18845-19684 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
TEM-135.v1 |
TEM-135.v1 |
Gene presence detected |
penicillin beta-lactam |
96_pilon |
991 |
2 |
844 |
861 |
19 |
861 |
99.88 |
97.91 |
CP117718.1:71328-72188 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
catA1 |
catA1 |
Gene presence detected |
phenicol antibiotic |
93_pilon |
1119 |
670 |
1119 |
660 |
211 |
660 |
99.78 |
68.18 |
NG_047582.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
ompK36 |
ompK36 |
p.135_136insD |
Inactivating mutation detected |
2_pilon |
438666 |
240738 |
241859 |
1104 |
1 |
1104 |
92.38 |
101.63 |
NC_016845.1:3727882-3728985 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
2024187392 |
ompK35 |
ompK35 |
c.T106del |
Inactivating mutation detected |
22_pilon |
93665 |
2 |
106 |
1080 |
1 |
105 |
100.00 |
9.75 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||||
2024187392 |
mgrB |
mgrB |
c.T50del |
Inactivating mutation detected |
peptide antibiotic |
11_pilon |
174550 |
2 |
50 |
144 |
1 |
49 |
100.00 |
34.04 |
NC_016845.1:3337154-3337297 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
2024187392 |
gyrA |
gyrA |
p.S83F |
Protein variant detected |
fluoroquinolone antibiotic |
2_pilon |
438666 |
230990 |
231350 |
360 |
1 |
360 |
99.44 |
100.00 |
HM560971.1:119269-119072 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
120 |
120 |
1 |
120 |
1 |
120 |
||
2024187392 |
gyrA |
gyrA |
p.D87N |
Protein variant detected |
fluoroquinolone antibiotic |
2_pilon |
438666 |
230990 |
231350 |
360 |
1 |
360 |
99.44 |
100.00 |
HM560971.1:119269-119072 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
120 |
120 |
1 |
120 |
1 |
120 |
||
2024187392 |
parC |
parC |
p.S80I |
Protein variant detected |
fluoroquinolone antibiotic |
24_pilon |
87413 |
57118 |
57478 |
360 |
1 |
360 |
99.72 |
100.00 |
HM560971.1:119269-119072 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
120 |
120 |
1 |
120 |
1 |
120 |
||
SRR33580217 |
SHV-11 |
SHV-11 |
Gene presence detected |
penicillin beta-lactam |
1 |
1055808 |
375169 |
376029 |
861 |
1 |
861 |
99.88 |
100.00 |
CP168408.1:2797577-2798437 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR33580217 |
blaSHV |
blaSHV |
p.L35Q |
Protein variant detected |
penicillin beta-lactam |
1 |
1055808 |
375169 |
376029 |
861 |
1 |
861 |
99.88 |
100.00 |
CP067381.1:82914-84074 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
SRR33580216 |
SHV-11 |
SHV-11 |
Gene presence detected |
penicillin beta-lactam |
1 |
539719 |
246594 |
247454 |
861 |
1 |
861 |
99.88 |
100.00 |
CP168408.1:2797577-2798437 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR33580216 |
blaSHV |
blaSHV |
p.L35Q |
Protein variant detected |
penicillin beta-lactam |
1 |
539719 |
246594 |
247454 |
861 |
1 |
861 |
99.88 |
100.00 |
CP067381.1:82914-84074 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
SRR5386028 |
aph(3)-Ia.v1 |
aph(3)-Ia.v1 |
Gene presence detected |
aminoglycoside antibiotic |
139 |
1042 |
88 |
903 |
816 |
1 |
816 |
99.88 |
100.00 |
CP157740.1:12584-13348 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
aadA2.v1 |
aadA2.v1 |
Gene presence detected |
aminoglycoside antibiotic |
89 |
4753 |
2301 |
3092 |
792 |
1 |
792 |
100.00 |
100.00 |
NG_047343.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
aac(6)-Ib-cr5 |
aac(6)-Ib-cr5 |
Gene presence detected |
aminoglycoside antibiotic |
113 |
2188 |
1541 |
2095 |
555 |
1 |
555 |
99.82 |
100.00 |
NG_051711.1:101-655 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
aac(6)-Ib-cr5 |
aac(6)-Ib-cr5 |
Gene presence detected |
Fluoroquinolone antibiotic |
113 |
2188 |
1541 |
2095 |
555 |
1 |
555 |
99.82 |
100.00 |
NG_051711.1:101-655 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
mrx |
mrx |
Gene presence detected |
macrolide antibiotic |
83 |
5960 |
3749 |
4987 |
1239 |
1 |
1239 |
100.00 |
100.00 |
LC613143.1:28805-30043 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
mphA |
mphA |
Gene presence detected |
macrolide antibiotic |
83 |
5960 |
4984 |
5889 |
906 |
1 |
906 |
100.00 |
100.00 |
NG_047985.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
sul1 |
sul1 |
Gene presence detected |
sulfonamide antibiotic |
89 |
4753 |
3597 |
4436 |
840 |
1 |
840 |
100.00 |
100.00 |
NG_048082.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
tet(D) |
tet(D) |
Gene presence detected |
tetracycline antibiotic |
97 |
3526 |
1642 |
2826 |
1185 |
1 |
1185 |
100.00 |
100.00 |
NG_051690.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
dfrA12 |
dfrA12 |
Gene presence detected |
trimethoprim antibiotic |
89 |
4753 |
1396 |
1893 |
498 |
1 |
498 |
100.00 |
100.00 |
NG_047689.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
TEM-1 |
TEM-1 |
Gene presence detected |
penicillin beta-lactam |
101 |
2693 |
148 |
1008 |
861 |
1 |
861 |
99.77 |
100.00 |
AY263331.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
OXA-1 |
OXA-1 |
Gene presence detected |
penicillin beta-lactam |
113 |
2188 |
580 |
1410 |
831 |
1 |
831 |
100.00 |
100.00 |
NG_049392.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
CTX-M-15 |
CTX-M-15 |
Gene presence detected |
third-generation cephalosporin |
92 |
4288 |
1701 |
2576 |
876 |
1 |
876 |
100.00 |
100.00 |
NG_048935.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
SHV-5 |
SHV-5 |
Gene presence detected |
third-generation cephalosporin |
3 |
232195 |
203475 |
204335 |
861 |
1 |
861 |
100.00 |
100.00 |
NG_050087.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
KPC-2 |
KPC-2 |
Gene presence detected |
carbapenem |
62 |
17724 |
11109 |
11990 |
882 |
1 |
882 |
100.00 |
100.00 |
NG_049253.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
OXA-9.v1 |
OXA-9.v1 |
Gene presence detected |
penicillin beta-lactam |
101 |
2693 |
1708 |
2547 |
840 |
1 |
840 |
99.88 |
100.00 |
CP067618.1:18845-19684 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
catB3.v1 |
catB3.v1 |
Gene presence detected |
phenicol antibiotic |
113 |
2188 |
1 |
442 |
633 |
1 |
442 |
100.00 |
69.83 |
NG_047605.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
blaSHV |
blaSHV |
p.G238S |
Gene presence detected |
third-generation cephalosporin |
3 |
232195 |
203475 |
204335 |
861 |
1 |
861 |
100.00 |
100.00 |
NG_050087.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
SRR5386028 |
blaSHV |
blaSHV |
p.E240K |
Gene presence detected |
third-generation cephalosporin |
3 |
232195 |
203475 |
204335 |
861 |
1 |
861 |
100.00 |
100.00 |
NG_050087.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
SRR5386028 |
ompK36 |
ompK36 |
c.25C>T |
Nucleotide variant detected |
2 |
276505 |
127569 |
128687 |
1104 |
1 |
1104 |
92.44 |
101.36 |
NC_016845.1:3727882-3728985 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
SRR5386028 |
mgrB |
mgrB |
p.K43Ter |
Inactivating mutation detected |
peptide antibiotic |
5 |
193631 |
83176 |
83319 |
144 |
1 |
144 |
99.31 |
89.36 |
NC_016845.1:3337154-3337297 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
SRR5386028 |
gyrA |
gyrA |
p.S83F |
Protein variant detected |
fluoroquinolone antibiotic |
2 |
276505 |
117822 |
118182 |
360 |
1 |
360 |
99.44 |
100.00 |
HM560971.1:119269-119072 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
120 |
120 |
1 |
120 |
1 |
120 |
||
SRR5386028 |
gyrA |
gyrA |
p.D87N |
Protein variant detected |
fluoroquinolone antibiotic |
2 |
276505 |
117822 |
118182 |
360 |
1 |
360 |
99.44 |
100.00 |
HM560971.1:119269-119072 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
120 |
120 |
1 |
120 |
1 |
120 |
||
SRR5386028 |
parC |
parC |
p.S80I |
Protein variant detected |
fluoroquinolone antibiotic |
7 |
156589 |
145285 |
145645 |
360 |
1 |
360 |
99.72 |
100.00 |
HM560971.1:119269-119072 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
120 |
120 |
1 |
120 |
1 |
120 |
||
ERR14097885 |
aph(3)-Ia.v1 |
aph(3)-Ia.v1 |
Gene presence detected |
aminoglycoside antibiotic |
76 |
1051 |
67 |
882 |
816 |
1 |
816 |
99.75 |
100.00 |
CP157740.1:12584-13348 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
aadA1.v2 |
aadA1.v2 |
Gene presence detected |
aminoglycoside antibiotic |
62 |
2006 |
136 |
924 |
789 |
1 |
789 |
100.00 |
100.00 |
NG_047327.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
aph(3)-VI |
aph(3)-VI |
Gene presence detected |
aminoglycoside antibiotic |
44 |
7811 |
1083 |
1862 |
780 |
1 |
780 |
100.00 |
100.00 |
NG_051730.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
armA |
armA |
Gene presence detected |
aminoglycoside antibiotic |
45 |
7423 |
6437 |
7210 |
774 |
1 |
774 |
100.00 |
100.00 |
NG_047476.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
qnrS1 |
qnrS1 |
Gene presence detected |
Fluoroquinolone antibiotic |
44 |
7811 |
5740 |
6396 |
657 |
1 |
657 |
100.00 |
100.00 |
NG_050543.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
msrE |
msrE |
Gene presence detected |
macrolide antibiotic |
45 |
7423 |
2663 |
4138 |
1476 |
1 |
1476 |
100.00 |
100.00 |
NG_048007.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
mrx |
mrx |
Gene presence detected |
macrolide antibiotic |
46 |
5939 |
3731 |
4969 |
1239 |
1 |
1239 |
99.92 |
100.00 |
LC613143.1:28805-30043 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
mphA |
mphA |
Gene presence detected |
macrolide antibiotic |
46 |
5939 |
4966 |
5871 |
906 |
1 |
906 |
100.00 |
100.00 |
NG_047985.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
mphE.v2 |
mphE.v2 |
Gene presence detected |
macrolide antibiotic |
45 |
7423 |
1723 |
2607 |
885 |
1 |
885 |
100.00 |
100.00 |
NG_064660.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
catB3.v2 |
catB3.v2 |
Gene presence detected |
phenicol antibiotic |
57 |
2443 |
1165 |
1797 |
633 |
1 |
633 |
100.00 |
100.00 |
NG_047604.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
arr-3 |
arr-3 |
Gene presence detected |
rifamycin antibiotic |
57 |
2443 |
1882 |
2334 |
453 |
1 |
453 |
100.00 |
100.00 |
NG_048581.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
sul1 |
sul1 |
Gene presence detected |
sulfonamide antibiotic |
67 |
1611 |
318 |
1157 |
840 |
1 |
840 |
100.00 |
100.00 |
NG_048082.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
sul2 |
sul2 |
Gene presence detected |
sulfonamide antibiotic |
48 |
5101 |
3806 |
4621 |
816 |
1 |
816 |
100.00 |
100.00 |
NG_051852.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
dfrA5 |
dfrA5 |
Gene presence detected |
trimethoprim antibiotic |
85 |
560 |
71 |
544 |
474 |
1 |
474 |
100.00 |
100.00 |
NG_051699.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
dfrA50 |
dfrA50 |
Gene presence detected |
trimethoprim antibiotic |
17 |
112548 |
12768 |
13244 |
477 |
1 |
477 |
98.95 |
100.00 |
NG_242637.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
OXA-9.v1 |
OXA-9.v1 |
Gene presence detected |
penicillin beta-lactam |
62 |
2006 |
969 |
1808 |
840 |
1 |
840 |
100.00 |
100.00 |
CP067618.1:18845-19684 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
OXA-1 |
OXA-1 |
Gene presence detected |
penicillin beta-lactam |
57 |
2443 |
197 |
1027 |
831 |
1 |
831 |
100.00 |
100.00 |
NG_049392.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
CTX-M-15 |
CTX-M-15 |
Gene presence detected |
third-generation cephalosporin |
59 |
2170 |
981 |
1856 |
876 |
1 |
876 |
100.00 |
100.00 |
NG_048935.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
NDM-5 |
NDM-5 |
Gene presence detected |
carbapenem |
54 |
3392 |
192 |
1004 |
813 |
1 |
813 |
100.00 |
100.00 |
NG_049337.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
SHV-11 |
SHV-11 |
Gene presence detected |
penicillin beta-lactam |
3 |
391795 |
276306 |
277166 |
861 |
1 |
861 |
99.88 |
100.00 |
CP168408.1:2797577-2798437 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
TEM-150 |
TEM-150 |
Gene presence detected |
penicillin beta-lactam |
43 |
8581 |
1 |
842 |
861 |
20 |
861 |
99.88 |
97.79 |
NG_050194.1 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
aac(6)-Ib-AKT.v3 |
aac(6)-Ib-AKT.v3 |
Gene presence detected |
aminoglycoside antibiotic |
87 |
521 |
1 |
489 |
555 |
1 |
489 |
100.00 |
88.11 |
NG_052418.1:101-655 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
blaSHV |
blaSHV |
p.L35Q |
Protein variant detected |
penicillin beta-lactam |
3 |
391795 |
276306 |
277166 |
861 |
1 |
861 |
99.88 |
100.00 |
CP067381.1:82914-84074 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
ERR14097885 |
ompK36 |
ompK36 |
p.134_135insGD |
Inactivating mutation detected |
11 |
198590 |
122632 |
123741 |
1104 |
1 |
1104 |
98.83 |
100.54 |
NC_016845.1:3727882-3728985 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
|||||||||
ERR14097885 |
ompK35 |
ompK35 |
p.R60LfsTer31 |
Inactivating mutation detected |
6 |
348123 |
178574 |
179660 |
1080 |
1 |
1080 |
99.26 |
25.07 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||||
ERR14097885 |
pmrB |
pmrB |
c.G445del |
Inactivating mutation detected |
peptide antibiotic |
6 |
348123 |
91 |
534 |
1098 |
1 |
444 |
99.55 |
40.55 |
NC_016845.1:1694393-1695491 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
||||||||
ERR14097885 |
gyrA |
gyrA |
p.S83I |
Protein variant detected |
fluoroquinolone antibiotic |
11 |
198590 |
112885 |
113245 |
360 |
1 |
360 |
99.44 |
100.00 |
HM560971.1:119269-119072 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
120 |
120 |
1 |
120 |
1 |
120 |
||
ERR14097885 |
parC |
parC |
p.S80I |
Protein variant detected |
fluoroquinolone antibiotic |
19 |
103230 |
72931 |
73291 |
360 |
1 |
360 |
99.44 |
100.00 |
HM560971.1:119269-119072 |
Kleborate |
3.2.4 |
Kleborate_AMRdb |
3.3.0 |
120 |
120 |
1 |
120 |
1 |
120 |
test/kpsc_test/example_output/klebsiella_pneumo_complex_genotype_spec.txt:
sample |
genotyping_method |
genotyping_schema_taxon |
genotyping_database_name |
genotyping_database_version |
genotyping_schema_name |
genotyping_software_name |
genotyping_software_version |
genotype |
genotype_confidence_value |
genotype_predicted_phenotype |
|---|---|---|---|---|---|---|---|---|---|---|
007_S09 |
In silico species detection |
Enterobacterales [NCBITaxon:91347] |
kleborate_enterobacterales__species |
3.2.4 |
enterobacterales__species |
Kleborate |
3.2.4 |
Klebsiella pneumoniae |
strong |
|
007_S09 |
MLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2024-12-31 |
MLST |
Kleborate |
3.2.4 |
ST11-1LV |
||
007_S09 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_K |
3.2.2 |
Kaptive |
3.3.1 |
KL64 |
Typeable |
Capsule null |
|
007_S09 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_O |
3.2.0 |
Kaptive |
3.3.1 |
OL2α.1 |
Typeable |
O2α |
|
007_S09 |
cgMLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
cgST766 |
||
007_S09 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
0_0_105_0_0_0_9_0_0_0 |
||
007_S09 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
SL258 |
||
007_S09 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
Cannot be determined |
||
2024187392 |
In silico species detection |
Enterobacterales [NCBITaxon:91347] |
kleborate_enterobacterales__species |
3.2.4 |
enterobacterales__species |
Kleborate |
3.2.4 |
Klebsiella pneumoniae |
strong |
|
2024187392 |
MLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2024-12-31 |
MLST |
Kleborate |
3.2.4 |
ST383 |
||
2024187392 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_K |
3.2.2 |
Kaptive |
3.3.1 |
KL30 |
Typeable |
K30 |
|
2024187392 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_O |
3.2.0 |
Kaptive |
3.3.1 |
OL2α.2 |
Typeable |
O2β |
|
2024187392 |
cgMLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
cgST19975; cgST20666; cgST52869; cgST63343 |
||
2024187392 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
0_0_87_5_0_3_1_0_0_0 |
||
2024187392 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
SL383 |
||
2024187392 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
CG383 |
||
SRR33580217 |
In silico species detection |
Enterobacterales [NCBITaxon:91347] |
kleborate_enterobacterales__species |
3.2.4 |
enterobacterales__species |
Kleborate |
3.2.4 |
Klebsiella pneumoniae |
strong |
|
SRR33580217 |
MLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2024-12-31 |
MLST |
Kleborate |
3.2.4 |
ST23 |
||
SRR33580217 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_K |
3.2.2 |
Kaptive |
3.3.1 |
KL1 |
Typeable |
K1 |
|
SRR33580217 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_O |
3.2.0 |
Kaptive |
3.3.1 |
OL2α.2 |
Typeable |
O2β |
|
SRR33580217 |
cgMLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
cgST65856 |
||
SRR33580217 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
0_0_429_0_44_1_19_0_0_0 |
||
SRR33580217 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
SL23 |
||
SRR33580217 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
CG23 |
||
SRR33580216 |
In silico species detection |
Enterobacterales [NCBITaxon:91347] |
kleborate_enterobacterales__species |
3.2.4 |
enterobacterales__species |
Kleborate |
3.2.4 |
Klebsiella pneumoniae |
strong |
|
SRR33580216 |
MLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2024-12-31 |
MLST |
Kleborate |
3.2.4 |
ST23 |
||
SRR33580216 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_K |
3.2.2 |
Kaptive |
3.3.1 |
KL1 |
Typeable |
K1 |
|
SRR33580216 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_O |
3.2.0 |
Kaptive |
3.3.1 |
OL2α.2 |
Typeable |
O2β |
|
SRR33580216 |
cgMLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
cgST65857 |
||
SRR33580216 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
0_0_429_0_4_0_35_0_0_0 |
||
SRR33580216 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
SL23 |
||
SRR33580216 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
CG23 |
||
SRR5386028 |
In silico species detection |
Enterobacterales [NCBITaxon:91347] |
kleborate_enterobacterales__species |
3.2.4 |
enterobacterales__species |
Kleborate |
3.2.4 |
Klebsiella pneumoniae |
strong |
|
SRR5386028 |
MLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2024-12-31 |
MLST |
Kleborate |
3.2.4 |
ST13 |
||
SRR5386028 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_K |
3.2.2 |
Kaptive |
3.3.1 |
KL3 |
Typeable |
K3 |
|
SRR5386028 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_O |
3.2.0 |
Kaptive |
3.3.1 |
OL2α.2 |
Typeable |
O2β |
|
SRR5386028 |
cgMLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
cgST32525 |
||
SRR5386028 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
0_0_98_0_11_2_0_1_1_0 |
||
SRR5386028 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
SL13 |
||
SRR5386028 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
CG13 |
||
ERR14097885 |
In silico species detection |
Enterobacterales [NCBITaxon:91347] |
kleborate_enterobacterales__species |
3.2.4 |
enterobacterales__species |
Kleborate |
3.2.4 |
Klebsiella pneumoniae |
strong |
|
ERR14097885 |
MLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2024-12-31 |
MLST |
Kleborate |
3.2.4 |
ST147 |
||
ERR14097885 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_K |
3.2.2 |
Kaptive |
3.3.1 |
KL64 |
Typeable |
K64 |
|
ERR14097885 |
In silico serotyping |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
Klebsiella_pneumoniae_Species_Complex_O |
3.2.0 |
Kaptive |
3.3.1 |
OL2α.1 |
Typeable |
O2α |
|
ERR14097885 |
cgMLST |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
cgST10000; cgST11351; cgST35980; cgST65374 |
||
ERR14097885 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
0_0_197_0_4_1_0_7_15_0 |
||
ERR14097885 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
SL147 |
||
ERR14097885 |
LIN code |
Klebsiella pneumoniae species complex [NCBITaxon:3390273] |
pubmlst_klebsiella_seqdef |
2026-04-29 |
scgMLST629_S |
MiST |
1.4.0 |
CG147 |